Mutagenesis & its effects

Intended learning outcomes
________________________________________
At the end of this lecture you should be able
1. Explain the relationship between changes in nucleotide and amino
sequences. (LO 10.1)
2. Describe the different types of mutational changes,
insertion, deletion. (LO 10.2)
3. -Predict and explain the effect that different
mutation, missense mutation, nonsense mutation, frameshift
10.3)
4 . Describe how spontaneous and induced mutations
5. Describe the process and the role of DNA
6. Explain the relationship between DNA damage and
to:
acid
e.g. point mutation,
mutations may have, e.g. silent
mutation. (LO
may occur. (LO 10.4)
repair. LO 10.6))
cancer. (LO 10.7)

Intended learning outcomes
________________________________________
At the end of this lecture you should be able
1. Explain the relationship between changes in nucleotide and amino
sequences. (LO 10.1)
2. Describe the different types of mutational changes,
insertion, deletion. (LO 10.2)
3. -Predict and explain the effect that different
mutation, missense mutation, nonsense mutation, frameshift
10.3)
4 . Describe how spontaneous and induced mutations
5. Describe the process and the role of DNA
6. Explain the relationship between DNA damage and
to:
acid
e.g. point mutation,
mutations may have, e.g. silent
mutation. (LO
may occur. (LO 10.4)
repair. LO 10.6))
cancer. (LO 10.7)

Types of Mutations
Point mutation : A point mutation is a
nucleotide to another.
? Transition :
A purine replaces a purine (A to G or G
replaces a pyrimidine (C to T or T to
? Transversion :
A purine replaces a pyrimidine or vice
Point mutation: can occurred in coding regions
non coding regions (splice site, promoter
substution in single
to A) or a pyrimidine
C.
versa (A or G to T or C.
(exons ) and in
regions)