Human papilloma virus (HPV) Diagnosis

Human papilloma
virus (Hpv) Diagnosis
Dr.Hadi Almosawi

Persistent infection with high risk human
papillomavirus (HPV) is the main cause of
cervical cancer and is a precursor to cervical
intraepithelial neoplasia (CIN)

LAB DIAGNOSIS
The important methods to diagnose HPV
infection are:
1-Colposcopy and acetic acid test
2-Biopsy
3-DNA test (PCR, Southern Blot
Hybridization, In Situ Hybridization)
4-Pap smear

Colposcopy
Colposcopy is the examination of the cervix,
vagina, and in some instances the vulva
after the application of acetic acid solution;
coupled with obtaining colposcopically
directed biopsies of all lesions suspected of
representing neoplasia

Acetic acid test
applying a 3–5% acetic acid–moistened
gauze pad for 5-10 minutes on suspected
lesions of the , cervix.
Inconspicuous, flat, genital lesions
dysplastic, and neoplastic tissues turn white
(acetowhite).

Biopsy
Excisional biopsy is recommended when
colposcopic appearances indicate high
grade abnormality, when low grade
colposcopic change is associated with
severe dyskaryosis or worse, or when a
lesion extends into the canal.

DNA techniques
Polymerase chain reaction
PCR is a selective target amplification assay
capable of exponential and reproducible
increase in the HPV sequences present in
biological specimens. The amplification
process can theoretically produce one billion
copies from a single double stranded DNA
molecule after 30 cycles of amplification